Due to popular request, day one (8th October 2019) of our CRISPR workshop is now open to all, free-of-charge. You will get to hear talks from experts and discuss with them about planning for a CRISPR experiment.
The first talk will cover the basics and focus on the general workflow of CRISPR. The second talk will be given by an expert from Singapore who will elaborate about how to work with small amounts of samples, or lowly expressed genes when doing CRISPR work. The third speaker will talk about how to pick a good gene to target, especially when you are interested in investigating a certain biological pathway. Lastly, the final speaker will walk you through the free online software programs and the A-Zs of designing a specific, efficient guide RNA for CRISPR.
CPD points will also be offered. Click here for the schedule of Day 1 and the full schedule or email [email protected] to find out more.
The first talk will cover the basics and focus on the general workflow of CRISPR. The second talk will be given by an expert from Singapore who will elaborate about how to work with small amounts of samples, or lowly expressed genes when doing CRISPR work. The third speaker will talk about how to pick a good gene to target, especially when you are interested in investigating a certain biological pathway. Lastly, the final speaker will walk you through the free online software programs and the A-Zs of designing a specific, efficient guide RNA for CRISPR.
CPD points will also be offered. Click here for the schedule of Day 1 and the full schedule or email [email protected] to find out more.
Schedule:
Day 1 (8th October 2019)
9.15 - 10.00 am Mastering the art of CRISPR
10.00 – 11.00 am Breakfast
11.00 - 12.30 pm CRISPR for precision medicine: The power of partitioning in droplet digital PCR
12.30 – 1.00 pm Forum session with the experts
1.00 - 2.00 pm Lunch
2.00 – 3.00 pm The search for genic pathways in CRISPR research
3.00 – 4.00 pm Unlocking the potential of genome editing: Designing CRISPR gRNAs for therapeutic and diagnostic purposes
4.00 - 5.30 pm Lab session (Hands-On)
CRISPR bioinformatics
- Choosing a suitable vector
- Designing a specific gRNA
- Determining off-targets
5.30 pm Tea break
Day 1 (8th October 2019)
9.15 - 10.00 am Mastering the art of CRISPR
10.00 – 11.00 am Breakfast
11.00 - 12.30 pm CRISPR for precision medicine: The power of partitioning in droplet digital PCR
12.30 – 1.00 pm Forum session with the experts
1.00 - 2.00 pm Lunch
2.00 – 3.00 pm The search for genic pathways in CRISPR research
3.00 – 4.00 pm Unlocking the potential of genome editing: Designing CRISPR gRNAs for therapeutic and diagnostic purposes
4.00 - 5.30 pm Lab session (Hands-On)
CRISPR bioinformatics
- Choosing a suitable vector
- Designing a specific gRNA
- Determining off-targets
5.30 pm Tea break
Day 2 (9th October 2019)
8.10 - 8.45 am Breakfast
8.45 – 12.30 pm Lab session (Hands-On)
CRISPR cloning
12.30 – 1.00 pm Molecular work after CRISPR cloning
1.00 - 2.00 pm Lunch
2.00 – 5.00 pm Lab session (Hands-On)
Continuation of CRISPR cloning
5.00 pm Tea break
8.10 - 8.45 am Breakfast
8.45 – 12.30 pm Lab session (Hands-On)
CRISPR cloning
12.30 – 1.00 pm Molecular work after CRISPR cloning
1.00 - 2.00 pm Lunch
2.00 – 5.00 pm Lab session (Hands-On)
Continuation of CRISPR cloning
5.00 pm Tea break
Day 3 (10th October 2019)
8.10 - 8.45 am Breakfast
8.45 – 9.15 am CRISPR validation using Sanger sequencing
9.15 – 1.00 pm Lab session (Hands-On)
Verification of CRISPR clones
1.00 - 2.00 pm Lunch
2.00 – 2.45 pm Efficient delivery of CRISPR
2.45 - 5.00 pm Lab session (Hands-On):
Transfection of CRISPR into target cells
5.00 pm Tea break
8.10 - 8.45 am Breakfast
8.45 – 9.15 am CRISPR validation using Sanger sequencing
9.15 – 1.00 pm Lab session (Hands-On)
Verification of CRISPR clones
1.00 - 2.00 pm Lunch
2.00 – 2.45 pm Efficient delivery of CRISPR
2.45 - 5.00 pm Lab session (Hands-On):
Transfection of CRISPR into target cells
5.00 pm Tea break
Day 4 (11th October 2019)
8.10 - 8.45 am Breakfast
8.45 – 9.45 am Downstream analysis of efficiency of CRISPR
9.45 – 12.45 pm Lab session (Hands-On):
Downstream analysis of CRISPR efficiency
- Microscopy
- Sanger sequencing
- qPCR (Demo)
12.45 - 2.30 pm Lunch
2.30 - 4.00 pm CRISPR/Cas systems for different functions
4.00 – 5.00 pm Discussion, certificate presentation, and closing ceremony
8.10 - 8.45 am Breakfast
8.45 – 9.45 am Downstream analysis of efficiency of CRISPR
9.45 – 12.45 pm Lab session (Hands-On):
Downstream analysis of CRISPR efficiency
- Microscopy
- Sanger sequencing
- qPCR (Demo)
12.45 - 2.30 pm Lunch
2.30 - 4.00 pm CRISPR/Cas systems for different functions
4.00 – 5.00 pm Discussion, certificate presentation, and closing ceremony